Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse Eotaxin 2 (also known as CCL24) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse CCL24 antibody. CCL24 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse CCL24 Antibody is added and binds to CCL24 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CCL24 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse CCL24. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm